The in vivo middle cerebral artery occlusion (MCAO) animal model and the in vitro OGD stroke model are used to study the mechanism of ischemic stroke induction by activating the NF-κB pathway, NLRP3 inflammasome, and triggering a vicious cycle of oxidative stress, leading to neurotoxicity [95,97]. The gene discussed is NFKB1; the disease is ischemic stroke.