Therefore, we investigated in a diet induced obesity (DIO) rat model (1) the physiological effects of the CB1 ligand, AM251, (2) the inflammatory profile of AM251 treatment (3) the effect of AM251 treatment on specific adipocyte genes in both WAT and BAT depots and (4) the effects of AM251 treatment and acute adiponectin exposure on fatty acid oxidative pathways in skeletal muscle. Here, CNR1 is linked to obesity due to melanocortin 4 receptor deficiency.