Pusnik et al. showed that SARS-CoV-2 infection-induced CD4+CD40L+ T-cells may support the development of S-reactive memory B-cells [22], in line with this we have shown the highest anti-RBD IgG production and the highest S-, M-, N-reactive CD4+CD40L+ T-cell frequency in the infection boosted group. This evidence concerns the gene CD40LG and infection.