All three wildtype target tumor cell lines used in this study (RMA-S, B16 and YAC-1) showed an expression of Gal-9 (Figure 3E, upper panel), and Lgals9-/- YAC-1 showed complete loss of Gal-9 expression upon evaluation by western blotting (Figure 3E, lower panel). Here, LGALS9 is linked to neoplasm.