Next, we examined the effects of AEG-1 and miR-221 in the invasion, migration, and cellular proliferation activity by transwell assays, wound healing, and cell viability assays (MTT) in miR-221 mimic/inhibitor-, AEG-1 siRNA-, with their corresponding control- transfected HCC cells in in vitro. This evidence concerns the gene MTDH and hepatocellular carcinoma.