Next, we analyzed the AEG-1 and miR-221 roles in their regulatory network, which regulates cell cycle, apoptosis, angiogenesis, and autophagy in miR-221 mimic-, miR-221 inhibitor-, AEG-1 siRNA-, and their corresponding controls-transfected HCC panel by qRT-PCR. This evidence concerns the gene MTDH and hepatocellular carcinoma.