Figure 4C,D show that the IHC staining degree of muc5ac in the model group was significantly reduced compared with the control group. Compared with the model group, the DOW-high and DLW-high group could improve the decreased secretion of muc5ac in the lacrimal gland acinar cells caused by dry eye (p < 0.05), but no significant improvement was found in the CsA group. This evidence concerns the gene MUC5AC and dry eye syndrome.