Finally, while constitutive deletion of S100A4 in mice restrains the stemness, size and number of tumors induced by DEN/CCL4 administration [150], depletion of S100A4+ stromal cells in liver-specific PTEN knockout mice reduces the stem-like properties of HCC cells, but did not prevent tumorigenesis [130]. Here, PTEN is linked to hepatocellular carcinoma.