Additionally, the gut microbiome, spatial TIME CD8 T-cell infiltration and tumor cell contact, myeloid inhibition of CD8 T-cells, immunosuppressive myeloid derived suppressor cells, inhibitory T-Reg cells, activated dendritic cells, intratumoral hypoxia, B-cells, cancer associated fibroblasts, all play an important aspect of the TIME [40]. The gene discussed is CD8A; the disease is cancer.