To further investigate the role of PKD family genes in CCA, we performed the wound healing assay in both TFK-1 and HuCCT1, treated with each siRNA for PKs of the PKD family (Figure 6A–C) and found that all siRNAs of PKD family members reduced 50% of wound closure (Figure 6D–F). The gene discussed is ARAF; the disease is cholangiocarcinoma.