Filtering by a further anemia targeting genes list (96 genes; Supplementary Methods) and taking into account the blood count/smear abnormalities, indicating mild spherocytosis (not available from sons 1–3), resulted in a SLC4A1 candidate variant (c.2210C>T p.(Ala737Val)), which was found heterozygous in the affected wife and daughter, but was absent in the index and son 1 (Figure S2). This evidence concerns the gene SLC4A1 and anemia (phenotype).