Overall, our data suggest that E2F1 up-regulates the expression of miRNA-206 and 210-5p in human VAT, situating the E2F1-miRNA-206/210-5p pathway as an addition to an intricate E2F1-regulated network in visceral adipose tissue, which may contribute to an obesity phenotype complicated by T2DM. Here, E2F1 is linked to obesity due to melanocortin 4 receptor deficiency.