Consistent with this notion, depletion of E2F1 and E2F2 by RNA interference in the PC3 prostate cancer cell line significantly raised the percentage of Serine 139-phosphorylated histone H2AX (γH2AX)-positive cells, indicative of an increased DNA damage signaling (Figure 1A, Supplementary Figure S2A,B). Here, E2F1 is linked to prostate cancer.