We assessed the role of E2F in the sustained upregulation of nucleotide synthesis genes upon 5-FU exposure by transfecting PC3 and DU145 prostate cancer cells with two different sets of siRNA oligos specific for E2F1 and E2F2, and 24 h later treated the cells with 5-FU or vehicle for 72 h. Here, E2F1 is linked to Familial prostate cancer.