Overall, 48 regions of interest (12 tumor-adjacent stroma and 12 tumor epithelium, 12 CD45+ tumor and 12 CD45+ tumor-adjacent stroma) were identified in 4 tumor samples from four different prostate cancer patients using florescence morphology markers of epithelial, stromal and immune cells (i.e., PanCK, αSMA, and CD45, respectively). This evidence concerns the gene ACTA1 and Familial prostate cancer.