ACTA1 and neoplasm: Overall, 48 regions of interest (12 tumor-adjacent stroma and 12 tumor epithelium, 12 CD45+ tumor and 12 CD45+ tumor-adjacent stroma) were identified in 4 tumor samples from four different prostate cancer patients using florescence morphology markers of epithelial, stromal and immune cells (i.e., PanCK, αSMA, and CD45, respectively).