Consequently, we determined the protein expression of HB-EGF, p-EGFR, p-AKT, and p-ERK in GBM cells by western blot and observed that ADAM8 knockdown reduced the expression of HB-EGF, p-EGFR, p-AKT, and p-ERK (Figure 4D,E). The gene discussed is HBEGF; the disease is glioblastoma.