To investigate the role of ADAM8 in TAMs recruitment in vitro, we constructed ADAM8 knockdown GBM cells (U87MG_shA8 and G1_shA8) and scramble controls (U87MG_scramble and G1_scramble) and co-cultured these cells with THP-1 derived macrophages using a standard protocol to obtain M0 macrophages. Here, ADAM8 is linked to glioblastoma.