The Crl: NU(NCr)-Foxn1 nude mice were sacrificed at the end of the study, and H&E staining of tumor tissue, spleen, and intestine was performed according to the standard protocol described in the “Histological studies of HK-1 nasopharyngeal HNSCC xenograft model” section. The gene discussed is FOXN1; the disease is head and neck squamous cell carcinoma.