The iCAF and myCAF were shown to be induced from pancreatic stellate cells (PSCs) in distinct culture conditions with PDAC cells: myCAF was induced by contact co-culture of quiescent PSC with the PDAC organoid, while iCAF was induced in the separated co-culture using transwell cell culture assays, which was consistent with the location in the tumor tissue, as described above, and considered as a paracrine of TGF-β and IL-1α from the PDAC cells. Here, TGFB1 is linked to neoplasm.