Further analysis of pathway enrichment among the upregulated genes in the paired and unpaired samples demonstrated enrichment in both paired and unpaired samples among their overlapped signaling pathways, including drug metabolism, MAPK, ErbB, calcium, cGMP-PKG, sphingolipid, PI3K-Akt, Cushing’s syndrome, HPV infection, and proteoglycans in cancers (p < 0.05) (Table 4). The gene discussed is AKT1; the disease is Cushing syndrome due to macronodular adrenal hyperplasia.