Immunoprecipitation of PTTG1 performed in samples derived from three frozen seminoma specimens (S1, S2, S3), obtained from the above-mentioned patients (P1–3), revealed that PTTG1 interacts specifically with ZEB1 in all samples analyzed, albeit to varying degrees, reflecting the inherent variability of human tumor tissues (Figure 5C). This evidence concerns the gene ZEB1 and neoplasm.