CD274 and neoplasm: The workflow included (i) selection of a mouse melanoma parental cell line responsive to exogenous IFNγ, including induction of MHC-I and PD-L1 expression, (ii) augmentation of tumor mutation burden through ultraviolet (UVR)-mutagenesis, (iii) post-UVR single-cell cloning, (iv) selection of immunogenic tumor clones through indirect CD8 T-cell recognition test, (v) in vivo tumor engraftment, and vi) evaluation of local and systemic immunological responses, including systemic memory.