We found that osteosarcoma cell lines have negligible KCNN1 expression compared to EwS cell lines (expression relative to GAPDH and YWHAZ: EwS: 0.02 ± 0.003, N = 3, n = 48; OS: 0.0001 ± 0.00002, N = 3, n = 22). This evidence concerns the gene KCNN1 and osteosarcoma.