To investigate the effects of the small molecule inhibitors of the IL1β signalling pathway, MLX01, VX765 and Pacritinib, on disseminated breast cancer metastasis to bone in vivo, E0771 Luc GFP bone homing cells were injected into the blood circulation of C57BL/6J mice via intra-cardiac injection, and metastatic outgrowth was compared with control (PBS treated mice) and mice treated with Anakinra. Here, IL1B is linked to breast cancer.