To clarify whether the overexpression of ERCC1 and radiation exposure following ERCC1 overexpression could affect the proliferation and migration ability of colorectal cancer cells, HCT116-Tet-on-ERCC1 and COLO205-Tet-on-ERCC1 were divided into no expression ERCC1-, overexpression ERCC1 (treated with 2 μg/mL), no expression ERCC1 combined with radiation exposure, and overexpression ERCC1 combined with radiation exposure; ATP Lite analysis was performed at 24 h and 48 h. Here, ERCC1 is linked to colorectal cancer.