In the cohort of patients with PC primarily treated with surgery (n = 9), blood concentrations of total EVs (median EVs/μL = 2483.7; CI 95% 1885.6–2984.4), as well as CD45+ (median EVs/μL = 233.3; CI 95% 218.4–357.1), PD-L1+ (median EVs/μL = 70.0; CI 95% 43.7–303.3), CD45+PD-L1+ (median EVs/μL = 57.6; CI 95% 21.3–126.0) and CD45-PD-L1+ EVs (median EVs/μL = 28.6; CI 95% 12.4–177.3) were in line with blood levels of EV populations observed in the overall PC cohort. The gene discussed is CD274; the disease is pachyonychia congenita.