To further validate the expression differences of SRSF family genes, we further performed pairwise analysis on 50 pairs of samples from the TCGA dataset and 199 pairs of samples from the ICGC dataset, and the results indicated that eight genes (SRSF1, SRSF2, SRSF3, SRSF6, SRSF7, SRSF9, SRSF11, SRSF12) were markedly more abundant in HCC than in normal liver tissue (Figure 1D,E). The gene discussed is SRSF6; the disease is hepatocellular carcinoma.