VCN-01 is an E1A 24 bp-deleted (for selective replication in Rb deficient tumors), and partially E3 deleted type 5 oncolytic adenovirus that expresses hyaluronidase for degradation of the tumor extracellular matrix (ECM), which is especially prominent in pancreatic adenocarcinoma. This evidence concerns the gene DHTKD1 and neoplasm.