PC3 and DU145 cells are AR negative, and although they do not represent the AR-expressing PCa phenotypes, both are androgen-independent and express, to varying degrees, several NED-associated proteins, such as neuron specific enolase (NSE), chromogranin A (CgrA), TUBB3 and others [11,12,13,14,15,16,17,18]. This evidence concerns the gene ENO2 and posterior cortical atrophy.