This study correlated AHSA1 overexpression with the mutation of TP53, CTNNB1, ARID1A, and poor clinical characteristics and prognosis in HCC patients and demonstrated that AHSA1 promoted the proliferation, invasion, metastasis, and EMT of HCC both in vitro and in vivo, and suggested that AHSA1 may be a carcinogenic gene in HCC. This evidence concerns the gene CTNNB1 and hepatocellular carcinoma.