Across in ovo treatment (Table 4), the expressions of IL-2, IL-6, IL-10, TGF-β4, TLR-4, 1α-hydroxylase, and VDR were significantly higher at 2 wk post-infection (28 doa) in comparison to their expression levels before coccidiosis infection (14 doa). The gene discussed is IL10; the disease is infection.