The current study had five goals: to assess (1) the feasibility of collecting saliva samples from horses and children to measure stress (cortisol) and relaxation (oxytocin); (2) whether salivary assays of cortisol as a physiological measure of stress/anxiety and oxytocin as a measure of relaxation/bonding changed both during each lesson and over the course of the ten weeks for horses and youth; (3) whether changes in anxiety symptoms, emotional regulation, and self-efficacy found in the first trial were comparable; and (4) whether fidelity to the program was reliable. Here, OXT is linked to Anxiety.