Indeed, limiting cell–cell interactions between NECTIN1-wild-type melanoma cells in transwell assays, either by seeding them at low density or by subjecting them to the chemotactic gradient before they could form a cell layer, increased their migratory capacity to a level similar to that of NECTIN1-knockout cells (Fig. 4d and Extended Data Fig. 5d), implying that the formation of adherens junctions inhibits migration. Here, NECTIN1 is linked to melanoma.