NECTIN1 and melanoma: Culture media supplemented with dextran-treated charcoal-stripped serum, which contains nutrients but is depleted in hormones and growth factors, triggered adherens junction formation between NECTIN1-wild-type, but not NECTIN1-deficient, A375 human melanoma cells to the same extent as serum-free media, as assessed by immunofluorescence (Extended Data Fig. 9a), pointing to the lack of growth factors or hormones as the likely cause of this difference.