NECTIN1 and melanoma: To evaluate whether the effects of NECTIN1 inactivation were specific to A375 cells, we downregulated NECTIN1 in five additional human melanoma cell lines spanning the spectrum of melanocytic differentiation (from AXLhigh/MITFlow to AXLlow/MITFhigh) and belonging to the major phenotypic subtypes of melanoma: undifferentiated, neural crest-like and melanocytic26 (Extended Data Fig. 4g).