To compare putative roles for the zebrafish pax3 genes in zRMS tumourigenesis, pax3a+/−;pax3b+/−;pax3a:EGFP zebrafish were used to generate pax3a:EGFP (used as control), pax3a−/−;pax3a:EGFP, pax3b−/−;pax3a:EGFP and pax3a−/−;pax3b−/−;pax3a:EGFP zebrafish lines (Fig. 3F–I), and analysed for putative differences in tumour onset, spatial location and growth rate. This evidence concerns the gene PAX3 and neoplasm.