RNAscope of RP tissue from ICC1-7 and from an extended independent validation cohort confirmed higher JAG1 expression in ICC/IDC compared to benign luminal epithelial cells, Gleason pattern 3, and Gleason pattern 4 non-ICC (NC) prostate cancer (Fig. 3d, e). The gene discussed is JAG1; the disease is intrahepatic cholangiocarcinoma.