Consistent with elevated JAG1 in ICC/IDC cancer cells, NOTCH target genes were significantly increased in endothelial cells in clusters 0 (HES1) and 22 (HES1 and HEY1) and SMC (HES4) located the ICC/IDC TME compared to benign prostate (Fig. 4e). This evidence concerns the gene HES4 and intrahepatic cholangiocarcinoma.