Cluster 3 cells expressed PRF1, GZMA, GZMH, TNF, and IFNG; however, expression of IFNG and TNF were lower in ICC/IDC-enriched compared to benign-enriched CD8+ T cells (Fig. 6l and Supplementary Fig. 8a)77. This evidence concerns the gene CD8A and intrahepatic cholangiocarcinoma.