EPCAM and intrahepatic cholangiocarcinoma: Following tissue isolation and histological confirmation by H&E, paired benign-enriched and ICC/IDC-enriched samples were single-cell disassociated, stained with DAPI and antibodies against CD45 (pan-immune marker) and EpCAM (pan-epithelial marker), tagged for multi-plex sequencing, and flow-sorted into three DAPI- (live) populations that were CD45+ (immune cells), EpCAM+ (epithelial cells), or CD45-EpCAM- (cells other than immune and epithelial cells such as endothelial cells, smooth muscle cells, fibroblasts, and nerve cells).