To confirm whether increased adipocyte lipolysis contributes to the development of hepatic steatosis following chronic liquid sucrose intake, we repeated our sucrose drinking paradigm in mice homozygous for a LoxP-modified Pnpla2 allele36 with or without hemizygosity for an adipose tissue-specific Cre recombinase (Adipoq)37. Here, PNPLA2 is linked to fatty liver disease.