We found that ZIKV replicated in multiple organs of 3- to 4-week-old A129 mice after infection, induced significantly higher levels of creatine-kinase (CK), CK-MB, lactate dehydrogenase (LDH), alpha-hydroxybutyrate dehydrogenase (α-HBDH), cardiac troponin T (cTnT), and cardiac troponin I (cTnI) and abnormal electrocardiogram (ECG) presentations that are the biomarkers for acute myocardial injury, decreased the gap junction protein Cx43, and altered the structure of the intercalated disc (ICD). The gene discussed is TNNI3; the disease is infection.