Finally, AKT-specific inhibitor (LY294002) was used to verify the effect of lncRNA MNX1-AS1/PPFIA4 on the proliferation and apoptosis of colorectal cancer in vivo. LY29004 significantly inhibited the tumorigenic ability of MNX1-AS1 and PPFIA4 (Supplementary Figures 1(a), 1(b), 1(d), and 1(e)). The gene discussed is AKT1; the disease is colorectal cancer.