We conducted pan-cancer analysis to explore the association between FDX1 and prognosis, genomic instability, RNA methylation modifications, immune infiltration and signaling pathway activity etc. Our experimental result validated the inhibitory effect of FDX1 in copper induced cell-death in liver hepatocellular carcinoma and non-small cell lung cancer cell lines. This evidence concerns the gene FDX1 and non-small cell lung carcinoma.