The relative expression level of the hub genes was reverified in melanoma cell lines (Figure 2F), and showed that HIF1A, IL1B, HMOX1, MMP3, CDKN2A and TIMP1 were upregulated, while PTEN, PRKCA, ATF3 and BRAF were downregulated in melanoma samples. This evidence concerns the gene MMP3 and melanoma.