The relative expression level of the hub genes was reverified in melanoma cell lines (Figure 2F), and showed that HIF1A, IL1B, HMOX1, MMP3, CDKN2A and TIMP1 were upregulated, while PTEN, PRKCA, ATF3 and BRAF were downregulated in melanoma samples. The gene discussed is PTEN; the disease is melanoma.