After calculating the ssGSEA score representing the activity of 31 m7G-regulated genes in the pan-cancer cohort, we found that the ssGSEA score was negatively correlated with higher infiltration of some immune cells especially Mast cells, T cells CD4 memory resting, T cells CD8 and B cells, these cell types have been shown by multiple studies to hinder tumor progression and promote a hyperimmune state of the tumor microenvironment and affect patient outcomes (Lichterman and Reddy, 2021; Lohr et al., 2013; Zhang et al., 2019b; Fu and Jiang, 2018; Wouters and Nelson, 2018). This evidence concerns the gene CD4 and cancer.