NF-κB pathway was initiatively trigged with the phosphorylation of IKK response to inflammation factor, following the phosphorylation and degradation of IκB and then release the p65 protein from the complex of p65/p115/IκB, and induced its translocation from cytoplasm to nucleus for activating the gene expression of inflammatory factor such as TNFα, IL-1β, and IL-6, consequently causing the development of chronic diseases including cancer, diabetes, and osteoarthritis. Here, TNF is linked to osteoarthritis.