In total, 420 DEGs between NF and IHD (238 upregulated and 182 downregulated) were identified, and the top 20 genes with the most significant upregulation and downregulation were plotted in a heatmap and the top 10 genes with the most significant upregulation and downregulation were marked in a volcano map (Figure 1A). Here, NFASC is linked to myocardial ischemia.