The contents of serum TNF-α, IL-10, MDA, BUN and Cr, and urine Cr and PRO were significantly increased (P < 0.05) in the model group compared with the control group, while serum SOD was significantly decreased (P < 0.05), indicating that the CI-AKI rat was seriously injured and the CI-AKI model was successfully established. The gene discussed is TNF; the disease is acute kidney injury.