Similarly, we observed that the administration of PF inhibited the activation of the leptin/LepRb-mediated PI3K/AKT pathway in PI-IBS rats, which was associated with reduced depression- and anxiety-like behaviors, suggesting that PF ameliorates the symptoms in PI-IBS rats by suppressing the leptin/LepRb downstream pathway. The gene discussed is AKT1; the disease is depressive disorder.