Furthermore, we validated the correlation of VAV1, RHOA, and ZC3HAV1 expression and immune cell infiltration in three single-cell sequencing HCC datasets of GSE140028_10x, GSE140228_Smatseq 2, and GSE98638 from TISCH database (Fig. 8G). This evidence concerns the gene ZC3HAV1 and hepatocellular carcinoma.