Then, the gene effect score of a variety of human HCC cell lines via the CRISPR method from DepMap database indicated a significant role of VAV1, RHOA, and ZC3HAV1 in the process of HCC vulnerability, since the scores from most cell lines were < 0 scores (Fig. 7G–I). The gene discussed is ZC3HAV1; the disease is hepatocellular carcinoma.