To verify the role of H19 in WDSW-induced NAFLD in HuRhKO mice, HuRhKO mice were injected with a recombinant adenovirus encoding an H19 shRNA or control adenovirus (GFP) while being fed ad libitum a WDSW for 4 weeks. The gene discussed is H19; the disease is metabolic dysfunction-associated steatotic liver disease.