The results of the mRNA expression of tight junction protein in jejunum was found that Occludin in the MCE group, MUC-2 in the PBC group and ZO-1, Occludin and MUC-2 in the MCE + PBC group were significantly increased (P < 0.05) (Fig. 7B). The gene discussed is MUC2; the disease is primary biliary cholangitis.