STAT3 and pachyonychia congenita: In addition to the suppression of IGF1, BCL2 and NFKB1, disclosed by the analysis of PC driver genes, abrogation of endogenous IL30, consistently inhibited, in both human PC cell lines, the expression of STAT3, as confirmed at the protein level by WB analysis (Fig. 5E, F) and by immunohistochemistry in IL30KO tumors (Fig. 5G), and the expression of mediators of monocyte/macrophages and granulocyte recruitment, such as CSF2 (28), CXCL1 [44], CXCL8 [45], IL1A [49], TNF [50] and, primarily CXCL5 (− 16.96 times in IL30KO-DU145, and − 29.50 times in IL30KO-PC3) (Fig. 5H).