In this study, among the exogenous tracers injected into the circulation, we found that EBD, which binds to albumin to mimic a protein tracer, was extravasated most readily into the ischemic hemisphere than the nonischemic hemisphere in mice subjected to dMCAO; in comparison, fluorescein less readily extravasated into the ischemic brain, and 10 kDa dextran-FITC did not appear to extravasate into the brain after cerebral ischemia. This evidence concerns the gene ALB and Cerebral ischemia.