The mRNA levels of RhoU, FOXO6, WNT4, ST6GALNAC1, AGR2, P4HTM, NELL2 and ALPP were significantly downregulated due to SNHG16 or EIF4A3 knockdown in ESCC cells according to RNA-Seq (Fig. 5B). The gene discussed is WNT4; the disease is esophageal squamous cell carcinoma.