To investigate the associated signal pathways and potential target genes involved in SNHG16–EIF4A3 regulation in ESCC, a high-resolution transcriptome microarray (Shengyin Biotech, Shanghai, China) after SNHG16 or EIF4A3 knockdown in ESCC cells was performed. The gene discussed is EIF4A3; the disease is esophageal squamous cell carcinoma.