PDCD1 and neoplasm: To detect whether the pre-existing anti-MAA clonotypes in the tumor expand following immune checkpoint inhibitor therapy or new ones are recruited from the circulation, we gathered TCRβ-seq data from 213 longitudinal samples from 3 cohorts of melanoma patients, containing 100 melanoma patients treated with anti-PD1 with or without anti-CTLA44,6,7.