ACTA1 and neoplasm: Confocal imaging revealed that in addition to preserving the fluorescence of EGFP+ tumor cells and perfused lectin-649 labeled endothelial cells, each of these distinct antigens (GFAP, αSMA, and Tuj1) was robustly detected by indirect immunofluorescence following EZ Clearing and cryosectioning (Figure 6B-D, Video 4).